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KMID : 0545120100200040732
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Journal of Microbiology and Biotechnology
2010 Volume.20 No. 4 p.732 ~ p.736
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Production of novel cell-associated tannase from newly isolated Serratia ficaria DTC
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Belur Prasanna D.
Gopal Mugeraya
Nirmala K.R.
Basavaraj N.
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Abstract
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Five strains of tannic acid degrading bacteria were isolated and identified by phenotypic characterization. All the five isolates showed cell-associated activity, where as only three showed extracellular activity. Serratia ficaria DTC showing highest cell-associated activity (0.29 U/l) was selected for further shake flask studies. Tannase synthesis was growth associated and reached the peak in the late stationary phase of growth. Organic nitrogen sources enhanced the tannase production. Peak tannase production of 0.56 U/l was recorded in the medium having the initial pH of 6. The pH and temperature optima of the enzyme were found to be 8.9 and 35˚C respectively. This is the first report of cell-associated activity in case of bacterial tannase. Cell-associated tannase of Serratia ficaria DTC could be industrially important from the perspective of its activity at broad temperature and pH range, its unusually high activity at pH 8.9.
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KEYWORD
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Cell-associated tannase, Extracellular tannase, Serratia ficaria
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